Last updated: 2023-05-15
Checks: 7 0
Knit directory: fitnessGWAS/
This reproducible R Markdown analysis was created with workflowr (version 1.7.0.4). The Checks tab describes the reproducibility checks that were applied when the results were created. The Past versions tab lists the development history.
Great! Since the R Markdown file has been committed to the Git repository, you know the exact version of the code that produced these results.
Great job! The global environment was empty. Objects defined in the global environment can affect the analysis in your R Markdown file in unknown ways. For reproduciblity it’s best to always run the code in an empty environment.
The command set.seed(20180914)
was run prior to running
the code in the R Markdown file. Setting a seed ensures that any results
that rely on randomness, e.g. subsampling or permutations, are
reproducible.
Great job! Recording the operating system, R version, and package versions is critical for reproducibility.
Nice! There were no cached chunks for this analysis, so you can be confident that you successfully produced the results during this run.
Great job! Using relative paths to the files within your workflowr project makes it easier to run your code on other machines.
Great! You are using Git for version control. Tracking code development and connecting the code version to the results is critical for reproducibility.
The results in this page were generated with repository version efa957c. See the Past versions tab to see a history of the changes made to the R Markdown and HTML files.
Note that you need to be careful to ensure that all relevant files for
the analysis have been committed to Git prior to generating the results
(you can use wflow_publish
or
wflow_git_commit
). workflowr only checks the R Markdown
file, but you know if there are other scripts or data files that it
depends on. Below is the status of the Git repository when the results
were generated:
Ignored files:
Ignored: .DS_Store
Ignored: .Rapp.history
Ignored: .Rhistory
Ignored: .Rproj.user/
Ignored: .httr-oauth
Ignored: .pversion
Ignored: analysis/.DS_Store
Ignored: code/.DS_Store
Ignored: code/Drosophila_GWAS.Rmd
Ignored: data/.DS_Store
Ignored: data/derived/
Ignored: data/input/.DS_Store
Ignored: data/input/.pversion
Ignored: data/input/dgrp.fb557.annot.txt
Ignored: data/input/dgrp2.bed
Ignored: data/input/dgrp2.bim
Ignored: data/input/dgrp2.fam
Ignored: data/input/huang_transcriptome/
Ignored: figures/.DS_Store
Ignored: old_analyses/.DS_Store
Untracked files:
Untracked: figures/Figure 2 edited.pptx
Untracked: figures/fig1.pdf
Untracked: figures/fig1_font.pdf
Untracked: figures/fig2_SNPs_manhattan_plot_edited.png
Untracked: old_analyses/Data for old analyses/
Untracked: old_analyses/eQTL_analysis.Rmd
Untracked: old_analyses/fitness_data.csv
Untracked: old_analyses/gcta_quant_genetics_OLD.Rmd
Untracked: old_analyses/quantitative_genetics_OLD_brms.Rmd
Unstaged changes:
Modified: code/main_paper_figures.Rmd
Modified: code/main_paper_figures.docx
Modified: code/pdf_supp_material.Rmd
Modified: code/pdf_supp_material.pdf
Modified: figures/fig2_SNPs_manhattan_plot.png
Modified: figures/fig3_boyle_plot.pdf
Modified: figures/fig4_mutation_load.pdf
Modified: figures/fig5_quartiles_plot.pdf
Modified: figures/fig6_antagonism_ratios.pdf
Modified: figures/fig7_models.pdf
Deleted: output/README.md
Note that any generated files, e.g. HTML, png, CSS, etc., are not included in this status report because it is ok for generated content to have uncommitted changes.
These are the previous versions of the repository in which changes were
made to the R Markdown
(analysis/raw_data_and_line_means.Rmd
) and HTML
(docs/raw_data_and_line_means.html
) files. If you’ve
configured a remote Git repository (see ?wflow_git_remote
),
click on the hyperlinks in the table below to view the files as they
were in that past version.
File | Version | Author | Date | Message |
---|---|---|---|---|
Rmd | efa957c | lukeholman | 2023-05-15 | wflow_publish("analysis/raw_data_and_line_means.Rmd") |
html | 7d3c2ef | lukeholman | 2023-05-15 | Build site. |
Rmd | 03df599 | lukeholman | 2023-05-15 | wflow_publish("analysis/raw_data_and_line_means.Rmd") |
html | 4fc3c4f | lukeholman | 2023-04-10 | Build site. |
Rmd | 501f3d4 | lukeholman | 2023-04-10 | wflow_publish("analysis/raw_data_and_line_means.Rmd") |
html | 2f5aeaf | lukeholman | 2023-04-10 | Build site. |
Rmd | fece779 | lukeholman | 2023-04-10 | wflow_publish("analysis/raw_data_and_line_means.Rmd") |
html | afeb987 | lukeholman | 2023-03-21 | Build site. |
Rmd | b14f397 | lukeholman | 2023-03-21 | wflow_publish("analysis/raw_data_and_line_means.Rmd") |
library(tidyverse)
library(DT)
# Create a function to build HTML searchable tables
my_data_table <- function(df, filename){
datatable(
df, rownames=FALSE,
autoHideNavigation = TRUE,
extensions = c("Scroller", "Buttons"),
options = list(
autoWidth = TRUE,
dom = 'Bfrtip',
deferRender=TRUE,
scrollX=TRUE, scrollY=1000,
scrollCollapse=TRUE,
buttons =
list('pageLength',
list(extend = 'csv',
filename = filename)),
pageLength = 10
)
)
}
# Load the raw data collected during the experiments (clean up in get_predicted_line_means.Rmd)
raw_female_fitness <- read_csv("data/input/female_fitness_CLEANED.csv") %>%
select(vial, everything()) %>%
rename_all(~ str_to_sentence(str_replace_all(.x, "[.]", " ")))
raw_male_fitness <- read_csv("data/input/male_fitness_CLEANED.csv") %>%
select(vial, everything(), -num.GFP.males) %>%
rename_all(~ str_to_sentence(str_replace_all(.x, "[.]", " ")))
# Load the predicted line means, as calculated in get_predicted_line_means.Rmd
predicted_line_means <- read_csv("data/derived/predicted_line_means.csv") %>%
mutate(female.fitness.early = round(female.fitness.early, 3),
female.fitness.late = round(female.fitness.late, 3),
male.fitness.early = round(male.fitness.early, 3),
male.fitness.late = round(male.fitness.late, 3)) %>%
rename_all(~ str_to_sentence(str_replace_all(.x, "[.]", " "))) %>%
select(-Block)
To facilitate data re-use, we here provide tables showing the raw
data (i.e. the measurements of male and female fitness that were
collected on each individual replicate vial), as well as the estimated
line means that were calculated in this script. Press the
CSV
button to download a particular table as a
.csv
file.
Vial
is a unique ID for each vial, which groups
together observations of the same group of 5 DGRP females whose fitness
was measured.Block
is the experimental block in which fitness was
measured.Line
gives the DGRP line number of the focal
females.Female fitness early
gives the number of L1 larvae
produced by the DGRP females, in the early-life measurement.Female fitness late
gives the number of L1 larvae
produced by the DGRP females, in the late-life measurement.my_data_table(raw_female_fitness,
"female_fitness_raw_data")
Vial
is a unique ID for each vial, which groups
together observations of the same group of 5 DGRP females whose fitness
was measured.Block
is the experimental block in which fitness was
measured.Line
gives the DGRP line number of the focal malesEarly male rival
gives the number of L1 larvae sired by
the rival GFP males, in the early-life measurement.Early male focal
gives the number of L1 larvae sired by
the focal DGRP males, in the early-life measurement.Late male rival
gives the number of L1 larvae sired by
the rival GFP males, in the late-life measurement.Late male focal
gives the number of L1 larvae sired by
the focal DGRP males, in the late-life measurement.my_data_table(raw_male_fitness,
"male_fitness_raw_data")
The four columns other than Line
give the line mean
fitness for each of the four fitness traits. Fitness was estimated from
Bayesian multivariate Poisson or Binomial models described in this script. The fitness values
were expressed on the scale of the linear predictor (and thus are
approximately normally distributed), and have been scaled to have a mean
of zero and variance of one. Thus, a line with mean fitness equal to 1.5
is 1.5 standard deviations above average, on the scale of the linear
predictor. Note that male fitness was not measured in
line_354
due to experimenter error.
my_data_table(predicted_line_means,
"line_mean_fitness_data")
sessionInfo()
R version 4.2.2 (2022-10-31)
Platform: x86_64-apple-darwin17.0 (64-bit)
Running under: macOS Big Sur ... 10.16
Matrix products: default
BLAS: /Library/Frameworks/R.framework/Versions/4.2/Resources/lib/libRblas.0.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.2/Resources/lib/libRlapack.dylib
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] DT_0.27 lubridate_1.9.2 forcats_1.0.0 stringr_1.5.0
[5] dplyr_1.1.0 purrr_1.0.1 readr_2.1.4 tidyr_1.3.0
[9] tibble_3.1.8 ggplot2_3.4.1 tidyverse_2.0.0 workflowr_1.7.0.4
loaded via a namespace (and not attached):
[1] tidyselect_1.2.0 xfun_0.37 bslib_0.4.2 colorspace_2.1-0
[5] vctrs_0.5.2 generics_0.1.3 htmltools_0.5.4 yaml_2.3.7
[9] utf8_1.2.2 rlang_1.0.6 jquerylib_0.1.4 later_1.3.0
[13] pillar_1.8.1 glue_1.6.2 withr_2.5.0 bit64_4.0.5
[17] lifecycle_1.0.3 munsell_0.5.0 gtable_0.3.1 htmlwidgets_1.6.1
[21] evaluate_0.20 knitr_1.42 tzdb_0.3.0 callr_3.7.3
[25] fastmap_1.1.1 crosstalk_1.2.0 httpuv_1.6.9 ps_1.7.2
[29] parallel_4.2.2 fansi_1.0.4 Rcpp_1.0.10 promises_1.2.0.1
[33] scales_1.2.1 cachem_1.0.7 vroom_1.6.1 jsonlite_1.8.4
[37] bit_4.0.5 fs_1.6.1 hms_1.1.2 digest_0.6.31
[41] stringi_1.7.12 processx_3.8.0 getPass_0.2-2 rprojroot_2.0.3
[45] grid_4.2.2 cli_3.6.0 tools_4.2.2 magrittr_2.0.3
[49] sass_0.4.5 crayon_1.5.2 whisker_0.4.1 pkgconfig_2.0.3
[53] ellipsis_0.3.2 timechange_0.2.0 rmarkdown_2.20 httr_1.4.5
[57] rstudioapi_0.14 R6_2.5.1 git2r_0.31.0 compiler_4.2.2